ERC PROJECT – Weakinteract


Our group is running an ERC project (Starting Grant to A. Loquet) for the period 2015-2020. The project is called Weakinteract, for “Weak interactions in self-organizations studied by NMR spectroscopy in the supramolecular solid-state“. This project is related to the development of solid-state NMR methods to tackle complex supramolecular assemblies in the fields of biology and chemistry.

The current results of this ERC project have been reported in several publications

NMR Spectroscopic Assignment of Backbone and Side-Chain Protons in Fully Protonated Proteins: Microcrystals, Sedimented Assemblies, and Amyloid Fibrils
Stanek J, Andreas LB, Jaudzems K, Cala D, Lalli D, Bertarello A, Schubeis T, Akopjana I, Kotelovica S, Tars K, Pica A, Leone S, Picone D, Xu ZQ, Dixon NE, Martinez D, Berbon M, El Mammeri N, Noubhani A, Saupe S, Habenstein B, Loquet A, Pintacuda G.
Angew Chem Int Ed Engl. 2016 Dec 12;55(50):15504-15509. doi: 10.1002/anie.201607084

Diversity of Amyloid Motifs in NLR Signaling in Fungi.
Loquet A, Saupe SJ.
Biomolecules. 2017 Apr 13;7(2). pii: E38. doi: 10.3390/biom7020038

Bacterial Filamentous Appendages Investigated by Solid-State NMR Spectroscopy.
Habenstein B, Loquet A.
Methods Mol Biol. 2017;1615:415-448. doi: 10.1007/978-1-4939-7033-9_29.

Coiled-coil oligomerization controls localization of the plasma membrane REMORINs.
Martinez D, Legrand A, Gronnier J, Decossas M, Gouguet P, Lambert O, Berbon M, Verron L, Grélard A, Germain V, Loquet A, Mongrand S, Habenstein B.
J Struct Biol. 2018 Feb 23. pii: S1047-8477(18)30046-7. doi: 10.1016/j.jsb.2018.02.003.

3D structure determination of amyloid fibrils using solid-state NMR spectroscopy.
Loquet A, El Mammeri N, Stanek J, Berbon M, Bardiaux B, Pintacuda G, Habenstein B.
Methods. 2018 Apr 6. pii: S1046-2023(17)30251-7. doi:10.1016/j.ymeth.2018.03.014

Identification of a novel cell death inducing domain reveals that fungal amyloid-controlled programmed cell death is related to necroptosis
Daskalov A, Habenstein B, Sabaté R, Berbon M, Martinez D, Chaignepain S, Coulary-Salin B, Hofmann K, Loquet A, Saupe SJ
Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2720-5. doi: 10.1073/pnas.1522361113

Lipid Internal Dynamics Probed in Nanodiscs.
Martinez D, Decossas M, Kowal J, Frey L, Stahlberg H, Dufourc EJ, Riek R, Habenstein B, Bibow S, Loquet A.
Chemphyschem. 2017 Jun 1. doi: 10.1002/cphc.201700450

Structural basis for plant plasma membrane protein dynamics and organization into functional nanodomains.
Gronnier J, Crowet JM, Habenstein B, Nasir MN, Bayle V, Hosy E, Platre MP, Gouguet P, Raffaele S, Martinez D, Grelard A, Loquet A, Simon-Plas F, Gerbeau-Pissot P, Der C, Bayer EM, Jaillais Y, Deleu M, Germain V, Lins L, Mongrand S.
Elife. 2017 Jul 31;6. pii: e26404. doi: 10.7554/eLife.26404

Detection of side-chain proton resonances of fully protonated biosolids in nano-litre volumes by magic angle spinning solid-state NMR.
Tolchard J, Pandey MK, Berbon M, Noubhani A, Saupe SJ, Nishiyama Y, Habenstein B, Loquet A.
J Biomol NMR. 2018 Mar;70(3):177-185. doi: 10.1007/s10858-018-0168-3

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy.
Loquet A, Tolchard J, Berbon M, Martinez D, Habenstein B.
J Vis Exp. 2017 Sep 17;(127). doi: 10.3791/55779.